Polyamine-mediated turnover of ornithine decarboxylase in Chinese-hamster ovary cells
نویسندگان
چکیده
منابع مشابه
Expression of human chromosome 2 ornithine decarboxylase gene in ornithine decarboxylase-deficient Chinese hamster ovary cells.
Ornithine decarboxylase (ODC) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and ODC is essential for cell proliferation. After screening 2.5 X 10(5) plaq...
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Ornithine decarboxylase (OIK ) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and OIK is essential for cell proliferation. After screening 2.5 x 10* plaqu...
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sequential events of the growth response under controlled conditions without the influence of a transforming or promoting stimulus. We have previously demonstrated in mitotically syn chronized CHO4cells that vitamin A treatment arrested cells in mid-G1 phase (16, 17). The inhibitory effect of vitamin A was a function of cell cycle position; cell cycle progression was blocked only when retinol w...
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Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2360351